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Author: Ricke, R
Author: Bielinsky, A
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Methods Article

Easy detection of chromatin binding proteins by the histone association assay

Robin Ricke1 and Anja-Katrin Bielinsky1*

1 Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota. Minneapolis, MN 55455. USA.

* To whom correspondence should be addressed: Anja-Katrin Bielinsky, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota. 321 Church Street SE, 6-155 Jackson Hall, Minneapolis, MN 55455. USA. Phone: 612-624-2469. Fax: 612-625-2163. Email: bielinsk@cbs.umn.edu

Biol. Proced. Online 2005;7:60-69. doi:10.1251/bpo106
Submitted: March 22, 2005; Accepted: April 14, 2005; Published: May 09, 2005.

Indexing terms: Chromatin; Cross-linking Reagents; Formaldehyde; Histones; Immunoprecipitation.

Abbreviations: ChIP, Chromatin Immunoprecipitation; HAA, Histone Association Assay; Mcm10, Minichromosome Maintenance Protein 10; Orc2, Origin Recognition Complex protein 2; WCE, Whole Cell Extract.


Figure 1 Enlarged

Fig. 1:

Schematic of the histone association assay. The histone association assay is an adaptation of chromatin immunoprecipitation. Chromatin bound proteins (red, yellow, and green circles) are detected independent of non-chromatin bound proteins (purple circles) based on their ability to co-precipitate with histones (core histones shown as light blue circles) in formaldehyde crosslinked (black X) cell extracts.

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