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Author: Chandra, PK
Author: Wikel, SK
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Methods Article

Analyzing ligation mixtures using a PCR based method

Prafulla K. Chandra1* and Stephen K. Wikel1

1 Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. Farmington, CT. USA.

* To whom correspondence should be addressed: Prafulla K. Chandra, Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. 263 Farmington Avenue, Farmington, CT 06030-3710. USA. Email: pchandra@uchc.edu

Biol. Proced. Online 2005;7:93-100. doi:10.1251/bpo108
Submitted: March 07, 2005; Accepted: May 20, 2005; Published: June 17, 2005.

Indexing terms: Ligation; Nucleic Acids; Polymerase Chain Reaction.


Figure 1 Enlarged

Fig. 1:

Strategy used for generating inserts for ligation. A. Generating inserts for 60S-L37 and 40S-L17 using PCR from Aedes salivary gland cDNA clones for TA and cohesive end cloning. CDS-III 3’ primer used as insert specific primer. B. pCR 2.1 plasmid vector (Invitrogen, Carlsbad, CA, USA) used for TA and cohesive end cloning (Map not to scale. Complete vector sequence and map can be found at www.invitrogen.com).

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