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Author: Chandra, PK
Author: Wikel, SK
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Methods Article

Analyzing ligation mixtures using a PCR based method

Prafulla K. Chandra1* and Stephen K. Wikel1

1 Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. Farmington, CT. USA.

* To whom correspondence should be addressed: Prafulla K. Chandra, Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. 263 Farmington Avenue, Farmington, CT 06030-3710. USA. Email: pchandra@uchc.edu

Biol. Proced. Online 2005;7:93-100. doi:10.1251/bpo108
Submitted: March 07, 2005; Accepted: May 20, 2005; Published: June 17, 2005.

Indexing terms: Ligation; Nucleic Acids; Polymerase Chain Reaction.


Figure 2 Enlarged

Fig. 2:

PCR products from ligation mixtures using vector specific primers M13-R & M-13F. Electrophoresis on 1.2 % agarose gel and staining with Ethidium Bromide. A: Band 1 derived from recombinants and Band 2 from relegated pCR 2.1 vector. Lane 2 & 3 has two types of recombinant specific products and both were used for calculation band intensity/amount. B: PCR product amounts calculated as corresponding band intensity (mean grey value) using ImageJ 1.33 software (http://rsb.info.nih.gov/ij).

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