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Author: Chandra, PK
Author: Wikel, SK
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Methods Article

Analyzing ligation mixtures using a PCR based method

Prafulla K. Chandra1* and Stephen K. Wikel1

1 Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. Farmington, CT. USA.

* To whom correspondence should be addressed: Prafulla K. Chandra, Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. 263 Farmington Avenue, Farmington, CT 06030-3710. USA. Email: pchandra@uchc.edu

Biol. Proced. Online 2005;7:93-100. doi:10.1251/bpo108
Submitted: March 07, 2005; Accepted: May 20, 2005; Published: June 17, 2005.

Indexing terms: Ligation; Nucleic Acids; Polymerase Chain Reaction.


Figure 3 Enlarged

Fig. 3:

Distribution of transformants and Lig-PCR products from ligation reactions. Graph showing the relative percentages of transformants for each ligation mixtures. Colonies counted after blue-white screening on X-gal media. Also, confirmed by PCR (data not shown). Error bars: < 5%. Chart A: Recombinant and vector specific transformants expressed as percentage. Chart B: Recombinant and vector specific Lig-PCR products expressed as percentage (shown for comparison). The charts represent data from three independent experiments. TA & Coh-60S & 40S: Transformants obtained from TA and Cohesive ligation samples for 60S-L37 and 40S-L17. Refer to Figure 2 for description of Ligation samples.

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