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Author: Chandra, PK
Author: Wikel, SK
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Methods Article

Analyzing ligation mixtures using a PCR based method

Prafulla K. Chandra1* and Stephen K. Wikel1

1 Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. Farmington, CT. USA.

* To whom correspondence should be addressed: Prafulla K. Chandra, Center for Microbial Pathogenesis, MC3710, School of Medicine, University of Connecticut Health Center. 263 Farmington Avenue, Farmington, CT 06030-3710. USA. Email: pchandra@uchc.edu

Biol. Proced. Online 2005;7:93-100. doi:10.1251/bpo108
Submitted: March 07, 2005; Accepted: May 20, 2005; Published: June 17, 2005.

Indexing terms: Ligation; Nucleic Acids; Polymerase Chain Reaction.


Figure 4 Enlarged

Fig. 4:

Determining insert orientations using Lig-PCR method. All PCR reactions (except controls) were carried out with the insert specific primer CDS III-3’ (Table 1) and either M13-F or M13-R. TA: TA ligation; Cohesive: Cohesive (EcoR I-Hind III) ligation. F: M13- forward primer; R: M13-reverse primer.

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