[Abstract]  [Full Text]  [PDF]


Display
[Abstract]
[Full Text]
PDF
Search in Pubmed
Author: Jud, C
Author: Albrecht, U
Related works

Methods Article

A guideline for analyzing circadian wheel-running behavior in rodents under different lighting conditions

Corinne Jud1, Isabelle Schmutz1, Gabriele Hampp1, Henrik Oster2 and Urs Albrecht1*

1 Department of Medicine, Division of Biochemistry, University of Fribourg. 1700 Fribourg. Switzerland.
2 Max-Planck-Institute for Experimental Endocrinology. 30625 Hannover. Germany.

* To whom correspondence should be addressed: Urs Albrecht, Department of Medicine, Division of Biochemistry, University of Fribourg. 1700 Fribourg. Switzerland. Phone: +41 (0)26 300 86 36. Email: urs.albrecht@unifr.ch

Biol. Proced. Online 2005;7:101-116. doi:10.1251/bpo109
Submitted: April 25, 2005; Accepted: June 20, 2005; Published: July 13, 2005.

Indexing terms: Photoperiod; Chronobiology; Circadian Rhythm; Mice.


Figure 2 Enlarged

Fig. 2:

Transients and Aftereffects. Transients (blue bars) can be caused by various treatments, such as the release of the animal into constant conditions (A) or a shift in the lighting regime (B). These transients usually persist for several days depending on the strength of the provoking signal. The white and black bar at the top of the scheme depicts light (12 h) and darkness (12 h), respectively. (C) An animal kept in constant darkness (DD) displays a stable free-running rhythm with a period length τ1 before it is subjected to a light pulse. This pulse leads to a phase shift and often provokes τ2, which is different from τ1, as an aftereffect. If the animal is left in DD long enough after this treatment, it will again display its old period length τ1. The red regression lines are drawn through the onsets before and after the light pulse to determine τ1 and τ2, respectively. The black bar at the top of the scheme represents constant darkness (24 h) conditions. DD, constant darkness; LD, light-dark cycle.

Disclaimer/Legal Copyright Privacy Policy

ISSN: 1480-9222
Article © by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2007 Biological Procedures Online.