A guideline for analyzing circadian wheel-running behavior in rodents under different lighting conditions

Display

Search in Pubmed

Author: Jud, C
Author: Albrecht, U
Related works

Methods Article

A guideline for analyzing circadian wheel-running behavior in rodents under different lighting conditions

Corinne Jud¹, Isabelle Schmutz¹, Gabriele Hampp¹, Henrik Oster² and Urs Albrecht¹^*

¹ Department of Medicine, Division of Biochemistry, University of Fribourg. 1700 Fribourg. Switzerland.
² Max-Planck-Institute for Experimental Endocrinology. 30625 Hannover. Germany.

* To whom correspondence should be addressed: Urs Albrecht, Department of Medicine, Division of Biochemistry, University of Fribourg. 1700 Fribourg. Switzerland. Phone: +41 (0)26 300 86 36. Email: urs.albrecht@unifr.ch

Biol. Proced. Online 2005;7:101-116. doi:10.1251/bpo109
Submitted: April 25, 2005; Accepted: June 20, 2005; Published: July 13, 2005.

Indexing terms: Photoperiod; Chronobiology; Circadian Rhythm; Mice.

Figure 7 Enlarged

Fig. 7:

Adaptation time and CT diagram. (A) Linear recording of wheel-running activity of a male wild type mouse (C57BL/6 x 129SV) under LD 12:12 conditions. Without prior wheel-running experience mice need two to three weeks to fully develop their wheel-running capacity. Only after this initial training (and entraining) phase experimental manipulations should be applied (X-axis: days of experiment; Y-axis wheel revolutions; black and white bars indicate dark and light phases, respectively; bin size for activity counts is 6 min). (B) Diagram comparing circadian time (CT) in degrees versus CT units.

Disclaimer/Legal Copyright Privacy Policy

ISSN: 1480-9222
Article © by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2007 Biological Procedures Online.