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Author: Xia, J
Author: Truant, R
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Methods Article

Practical three color live cell imaging by widefield microscopy

Jianrun Xia1, Song Hon H. Kim1, Susan Macmillan1 and Ray Truant1*

1 Department of Biochemistry and Biomedical Sciences, McMaster University. HSC 4H24A 1200 Main Street West, Hamilton, Ontario, L8N 3Z5. Canada.

* To whom correspondence should be addressed: Ray Truant, Department of Biochemistry and Biomedical Sciences, McMaster University. HSC 4H24A 1200 Main Street West, Hamilton, Ontario, L8N 3Z5. Canada. Phone: 01-905-525-9140 ext. 22450. Fax: 01-905-522-9033. Email: truantr@mcmaster.ca

Biol. Proced. Online 2006;8:63-68. doi:10.1251/bpo119
Submitted: April 10, 2006; Accepted: June 13, 2006; Published: July 21, 2006.

Indexing terms: Green Fluorescent Proteins; Blue Fluorescent Protein, Aequorea Victoria.


Figure 1 Enlarged

Fig. 1:

PSCFP2 Can be Distinctly Imaged with a DAPI/hoechst Filter Set. Panels a-d, Transient transfection of 1ug each eGFP-ASF, PSCFP2-Mito localization signal, mRFP-huntingtin Exon1 Q138 fragment after 24 hours in NIH3T3 (non-plasmid replicating) cells. Less than 200 msec required for each channel of exposure. Scale bar is 10 um. Panels e-I, live cell four color imaging of PSCFP2-NXF1, mCerProfilin1, eYFP-Synapsin1, and mRFP-huntingtin exon1Q138. Distinct signals could be detected for each of the proteins. 4 channel color images and merge were assembled in Imaris 4.3 (Bitplane, Zurich, Switzerland). Scale bar is 10 μm.

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