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Author: Ray, S
Author: Das, SK
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Methods Article

Chromatin immunoprecipitation assay detects ERα recruitment to gene specific promoters in uterus

Sanhita Ray1 and Sanjoy K. Das1*

1 Departments of Pediatrics and Cancer Biology, Vanderbilt University Medical Center. Nashville, TN 37232. USA.

* To whom correspondence should be addressed: Sanjoy K. Das, Division of Reproductive and Developmental Biology, D-4105 Medical Center North, Department of Pediatrics, Vanderbilt University Medical Center, 1161 21st Avenue South. Nashville, TN 37232-2678. USA. Phone: 615-322-8644. Fax: 615-322-8397. Email: sanjoy.das@vanderbilt.edu

Biol. Proced. Online 2006;8:69-76. doi:10.1251/bpo120
Submitted: May 02, 2006; Accepted: July 12, 2006; Published: July 31, 2006.

Indexing terms: Chromatin; Immunoprecipitation; Estrogen Receptor Alpha.

Abbreviations: ChIP, chromatin immunoprecipitation; ERα, estrogen receptor-alpha; Ltf, lactoferrin; Pgr, progesterone receptor; Ccnd1, cyclin D1; IP, immunoprecipitation.


Figure 3 Enlarged

Fig. 3:

Analysis of recruitment of ERα to estrogen-responsive Ltf, Pgr and Ccnd1 gene promoters. ChIP analysis was performed using ERα antibody or normal serum IgG (as control) as described in materials and methods. The presence of the promoter DNA before immunoprecipitation was confirmed by PCR (Input). PCR products were resolved in 2% agarose gels containing ethidium bromide.

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