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Author: Ray, S
Author: Das, SK
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Methods Article

Chromatin immunoprecipitation assay detects ERα recruitment to gene specific promoters in uterus

Sanhita Ray1 and Sanjoy K. Das1*

1 Departments of Pediatrics and Cancer Biology, Vanderbilt University Medical Center. Nashville, TN 37232. USA.

* To whom correspondence should be addressed: Sanjoy K. Das, Division of Reproductive and Developmental Biology, D-4105 Medical Center North, Department of Pediatrics, Vanderbilt University Medical Center, 1161 21st Avenue South. Nashville, TN 37232-2678. USA. Phone: 615-322-8644. Fax: 615-322-8397. Email: sanjoy.das@vanderbilt.edu

Biol. Proced. Online 2006;8:69-76. doi:10.1251/bpo120
Submitted: May 02, 2006; Accepted: July 12, 2006; Published: July 31, 2006.

Indexing terms: Chromatin; Immunoprecipitation; Estrogen Receptor Alpha.

Abbreviations: ChIP, chromatin immunoprecipitation; ERα, estrogen receptor-alpha; Ltf, lactoferrin; Pgr, progesterone receptor; Ccnd1, cyclin D1; IP, immunoprecipitation.


Figure 4 Enlarged

Fig. 4:

Analysis of mRNA expression for Ltf, Pgr and Ccnd1 genes. RT-PCR analysis of Ltf, Pgr and Ccnd1 genes in the uterus. Total RNA was extracted and analyzed by comparative RT-PCR (21). Ribosomal protein L7 (rpl7) was used as a constitutive gene. Amplified DNA bands were visualized by ethidium bromide staining.

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