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Author: Badtke, MP
Author: Tavis, JE
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Methods Article

Combining genetic and biochemical approaches to identify functional molecular contact points

Matthew P. Badtke1, Feng Cao1 and John E. Tavis2*

1 Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine. St. Louis, MO 63104. USA.
2 Department of Molecular Microbiology and Immunology and Saint Louis University Liver Center, Saint Louis University School of Medicine. St. Louis, MO 63104. USA.

* To whom correspondence should be addressed: John E. Tavis, Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology. 1402 S. Grand Blvd., St. Louis, MO 63104. USA. Phone: 615-322-8644. Fax: 615-322-8397. Email: tavisje@slu.edu

Biol. Proced. Online 2006;8:77-86. doi:10.1251/bpo121
Submitted: May 02, 2006; Accepted: July 19, 2006; Published: August 10, 2006.

Indexing terms: Hepatitis B Virus, Duck; Protein Interaction Mapping.


Figure 1 Enlarged

Fig. 1:

Summary of how differential exposure of mAb epitopes can lead to identification of protein-protein contacts. (A) Under normal conditions only some of the antibodies can bind their epitopes due to occlusion of the epitopes by the ligand bound to the contact site. (B) Treatment with partially denaturing buffer dissociates the protein contact site, exposing the monoclonal antibody epitopes. Mutagenesis (C) and peptide competition (D) at the contact site can both disrupt binding of the ligand for the motif and expose the epitopes in a pattern similar to that induced by the partially denaturing buffer.

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