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Author: Badtke, MP
Author: Tavis, JE
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Methods Article

Combining genetic and biochemical approaches to identify functional molecular contact points

Matthew P. Badtke1, Feng Cao1 and John E. Tavis2*

1 Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine. St. Louis, MO 63104. USA.
2 Department of Molecular Microbiology and Immunology and Saint Louis University Liver Center, Saint Louis University School of Medicine. St. Louis, MO 63104. USA.

* To whom correspondence should be addressed: John E. Tavis, Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology. 1402 S. Grand Blvd., St. Louis, MO 63104. USA. Phone: 615-322-8644. Fax: 615-322-8397. Email: tavisje@slu.edu

Biol. Proced. Online 2006;8:77-86. doi:10.1251/bpo121
Submitted: May 02, 2006; Accepted: July 19, 2006; Published: August 10, 2006.

Indexing terms: Hepatitis B Virus, Duck; Protein Interaction Mapping.


Figure 5 Enlarged

Fig. 5:

Mutations can alter partial proteolysis pattern. P was partially digested with papain and the fragments were resolved by SDS-PAGE. Lanes 1-4 show wild-type P and lanes 5-8 show P with mutations which disrupt the contact site on P. ε is a RNA stem loop required for proper folding and activation of P. Note the lack of the protected fragment in the digestion of mutant protein (compare lanes 4 and 8).

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