[Abstract]  [Full Text]  [PDF]

Display
[Abstract]
[Full Text]
PDF
Search in Pubmed
Author: Badtke, MP
Author: Tavis, JE
Related works

Methods Article

Combining genetic and biochemical approaches to identify functional molecular contact points

Matthew P. Badtke1, Feng Cao1 and John E. Tavis2*

1 Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine. St. Louis, MO 63104. USA.
2 Department of Molecular Microbiology and Immunology and Saint Louis University Liver Center, Saint Louis University School of Medicine. St. Louis, MO 63104. USA.

* To whom correspondence should be addressed: John E. Tavis, Saint Louis University School of Medicine, Department of Molecular Microbiology and Immunology. 1402 S. Grand Blvd., St. Louis, MO 63104. USA. Phone: 615-322-8644. Fax: 615-322-8397. Email: tavisje@slu.edu

Biol. Proced. Online 2006;8:77-86. doi:10.1251/bpo121
Submitted: May 02, 2006; Accepted: July 19, 2006; Published: August 10, 2006.

Indexing terms: Hepatitis B Virus, Duck; Protein Interaction Mapping.


Figure 7 Enlarged

Fig. 7:

Soluble peptides containing the putative binding site can competitively inhibit protein function. P was translated in vitro in the presence of the T3 peptide or irrelevant MBP peptide. Priming was initiated by addition of MgCl2 and [α-32P]dGTP. Priming signal was normalized to the activity of P without DMSO or peptide. Peptide concentration is in mM and error bars are the standard deviation from 4 experiments. This figure was originally published in (9).

Disclaimer/Legal Copyright Privacy Policy

ISSN: 1480-9222
Article © by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2007 Biological Procedures Online.