A method for detecting functional activity related expression in gross brain regions, specific brain nuclei and individual neuronal cell bodies and their projections

Display

Search in Pubmed

Author: Murphy, M
Author: Wilson, YM
Related works

Methods Article

A method for detecting functional activity related expression in gross brain regions, specific brain nuclei and individual neuronal cell bodies and their projections

Mark Murphy¹^*, Ursula Greferath¹ and Yvette M. Wilson¹

¹ Department of Anatomy and Cell Biology, University of Melbourne. Melbourne, Victoria, 3010. Australia.

* To whom correspondence should be addressed: Mark Murphy, Department of Anatomy and Cell Biology, University of Melbourne. Melbourne, Victoria, 3010. Australia. Email: m.murphy@unimelb.edu.au

Biol. Proced. Online 2007;9:1-8. doi:10.1251/bpo128
Submitted: August 30, 2006; Accepted: October 18, 2006; Published: February 20, 2007.

Indexing terms: Mice, Transgenic; Brain Chemistry; beta-Galactosidase.

Figure 1 Enlarged

Fig. 1:

Examples of different modes of detection of activated FTL expression in brains of FTL mice. (A) and (B) show detection of FTL expression in neurons using βgal histochemistry. (A) shows a low power view of a coronal section of the brain (approximate Bregma - 1 mm) from an FTL mouse which had received a mild aversive stimulus (all sections were from mice which had received a footshock of 0.5 mA for 2 seconds). (B) shows higher power view of region boxed in (A), and which shows the basolateral complex of the amygdala. (C) and (D) shows detection of FTL expression in neurons using βgal peroxidase immunohistochemistry. (C) shows similar view as (B), and contains basolateral complex of amygdala. (D) shows higher power view of region boxed in (C). (E) shows FTL expression in dentate gyrus neurons using βgal fluorescence immunohistochemistry. Scale bars: A, 2 mm; B and C, 400 μm; D, 50 μm; E. 100 μm.

Disclaimer/Legal Copyright Privacy Policy