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Author: Koo, SH
Author: Lee, EJD
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Methods Article

Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array

Seok Hwee Koo1, Tan Ching Ong2, Kok Ting Chong1, Caroline Guat Lay Lee3, Fook Tim Chew2 and Edmund Jon Deoon Lee1*

1 Department of Pharmacology, National University of Singapore. Singapore.
2 Department of Biological Sciences, National University of Singapore. Singapore.
3 Department of Biochemistry, National University of Singapore. Block MD11, Level 5, #05-09, Clinical Research Centre, 10 Medical Drive. Singapore 117597. Phone: +65-65168437 Fax: +65-68737690 Email: edlee@nus.edu.sg.

* To whom correspondence should be addressed: Edmund Jon Deoon Lee, Department of Pharmacology, National University of Singapore. Singapore.

Biol. Proced. Online 2007;9:27-42. doi:10.1251/bpo131
Submitted: March 12, 2007; Accepted: July 3, 2007; Published: November 1, 2007.

Indexing terms: Genotype; Microspheres; Polymorphism, Genetic.

Abbreviations: ABC, ATP-binding cassette; ASPE, allele-specific primer extension; EDC, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; MES, 2[N-Morpholino] ethanesulfonic acid; MFI, median fluorescence intensity; SAP, shrimp alkaline phosphatase; SDS, sodium dodecyl sulfate; SNP, single nucleotide polymorphism.


Figure 1 Enlarged

Fig. 1:

Schematic diagram of ASPE reaction.

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ISSN: 1480-9222 Article © by Biological Procedures Online. This paper is Open Access. Copying, printing, redistribution and storage permitted. Journal © 1997-2007 Biological Procedures Online.