Assessment of different protocols for the isolation and purification of gut associated lymphoid cells from the gilthead seabream (Sparus aurata L.)

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Author: Salinas, I
Author: Esteban, M
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Methods Article

Assessment of different protocols for the isolation and purification of gut associated lymphoid cells from the gilthead seabream (Sparus aurata L.)

Irene Salinas¹, José Meseguer¹ and Maria Ángeles Esteban¹^*

¹ Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia. 30100 Murcia. Spain. Phone: +34 968367665 Fax: +34 968363963 Email: aesteban@um.es.

* To whom correspondence should be addressed: Maria Ángeles Esteban, Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia. 30100 Murcia. Spain. Phone: +34 968367665. Fax: +34 968363963. Email: aesteban@um.es

Biol. Proced. Online 2007;9:43-55. doi:10.1251/bpo132
Submitted: February 15, 2007; Accepted: March 30, 2007; Published: November 25, 2007.

Indexing terms: Sea Bream; Flow Cytometry; Respiratory Burst.

Figure 2 Enlarged

Fig. 2:

Scanning electron micrographs of nylon wool fibers from nylon wool columns incubated with seabream gut cells. (a) Mucus (arrow) from seabream gut trapped between nylon wool fibres. Bar: 200 μm (b) Small rounded cell (arrow head) adhered to a nylon wool fibre. Bar: 20 μm.

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