Assessment of different protocols for the isolation and purification of gut associated lymphoid cells from the gilthead seabream (Sparus aurata L.)

Display

Search in Pubmed

Author: Salinas, I
Author: Esteban, M
Related works

Methods Article

Assessment of different protocols for the isolation and purification of gut associated lymphoid cells from the gilthead seabream (Sparus aurata L.)

Irene Salinas¹, José Meseguer¹ and Maria Ángeles Esteban¹^*

¹ Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia. 30100 Murcia. Spain. Phone: +34 968367665 Fax: +34 968363963 Email: aesteban@um.es.

* To whom correspondence should be addressed: Maria Ángeles Esteban, Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia. 30100 Murcia. Spain. Phone: +34 968367665. Fax: +34 968363963. Email: aesteban@um.es

Biol. Proced. Online 2007;9:43-55. doi:10.1251/bpo132
Submitted: February 15, 2007; Accepted: March 30, 2007; Published: November 25, 2007.

Indexing terms: Sea Bream; Flow Cytometry; Respiratory Burst.

Figure 3 Enlarged

Fig. 3:

Purification of seabream GALT cells by Percoll density gradients. (a) Representative flow cytometry dot plot of intermediate density band (ID) corresponding to 1.060 g/l. (b) Representative flow cytometry dot plot of cells found at the highest density band (HD) (1.075 g/l). (c) Light micrograph of a Giemsa stained cytocentrifugation of ID band cells. (d) Light micrograph of a Giemsa stained cytocentrifugation of HD band cells.

Disclaimer/Legal Copyright Privacy Policy