An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development

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Author: Patel, N
Author: Rameshwar, P
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Methods Article

An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development

Nitixa Patel¹, Marianne Castillo¹ and Pranela Rameshwar¹^*

¹ Department of Medicine, UMDNJ-New Jersey Medical School, Newark, NJ. UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA. New Jersey. Phone: +(973) 972 0625 Fax: +(973) 972 8854 Email: rameshwa@umdnj.edu.

* To whom correspondence should be addressed: Rameshwar Pranela, Department of Medicine, UMDNJ-New Jersey Medical School, Newark, NJ. UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA. New Jersey. Phone: +(973) 972 0625. Fax: +(973) 972 8854. Email: rameshwa@umdnj.edu

Biol. Proced. Online 2007;9:56-64. doi:10.1251/bpo133
Submitted: May 25, 2007; Accepted: September 17, 2007; Published: December 14, 2007.

Indexing terms: cell culture techniques; hematopoiesis; cytokines; hematopoietic stem cells.

Abbreviations: BM, Bone marrow; CLP, common lymphoid progenitors; CMP, common myeloid progenitors; HSCs, hematopoietic stem cells; MSCs, mesenchymal stem cells; NK-A, neurokinin-A; NK1, neurokinin-1; NK2, neurokinin-2; SDF-1α, Stromal cell-derived factor 1α; SP, Substance P.

Figure 1 Enlarged

Fig. 1:

Scatchard analysis of SP-binding sites on CD34+ cells. We performed ligand-binding studies with 125I-Tyr-SP to confirm SP is capable of binding to CD34+ cells and also to determine the binding kinetics of SP on these cells.

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