An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development

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Author: Patel, N
Author: Rameshwar, P
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Methods Article

An in vitro method to study the effects of hematopoietic regulators during immune and blood cell development

Nitixa Patel¹, Marianne Castillo¹ and Pranela Rameshwar¹^*

¹ Department of Medicine, UMDNJ-New Jersey Medical School, Newark, NJ. UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA. New Jersey. Phone: +(973) 972 0625 Fax: +(973) 972 8854 Email: rameshwa@umdnj.edu.

* To whom correspondence should be addressed: Rameshwar Pranela, Department of Medicine, UMDNJ-New Jersey Medical School, Newark, NJ. UMDNJ-New Jersey Medical School, MSB, Rm. E-579 185 South Orange Ave, Newark, NJ 07103, USA. New Jersey. Phone: +(973) 972 0625. Fax: +(973) 972 8854. Email: rameshwa@umdnj.edu

Biol. Proced. Online 2007;9:56-64. doi:10.1251/bpo133
Submitted: May 25, 2007; Accepted: September 17, 2007; Published: December 14, 2007.

Indexing terms: cell culture techniques; hematopoiesis; cytokines; hematopoietic stem cells.

Abbreviations: BM, Bone marrow; CLP, common lymphoid progenitors; CMP, common myeloid progenitors; HSCs, hematopoietic stem cells; MSCs, mesenchymal stem cells; NK-A, neurokinin-A; NK1, neurokinin-1; NK2, neurokinin-2; SDF-1α, Stromal cell-derived factor 1α; SP, Substance P.

Figure 2 Enlarged

Fig. 2:

The effects of SP, NK-A and SDF-1α on hematopoiesis. BM stroma is innervated by peptidergic fibers, which can release two neuropeptides, SP and NK-A. SP interacts with BM Stroma via a specific receptor, NK1, which induces growth factors that stimulate hematopoiesis. NKA can also be produced by peptidergic fibers, which interact with NK2 on BM Stroma, which causes inhibition of hematopoiesis. High levels of SDF-1α stimulate BM Stroma to induced SP production, which stimulates hematopoiesis.

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