Improving image analysis in 2DGE-based redox proteomics by labeling protein carbonyl with fluorescent hydroxylamine

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Author: Poon, HF
Author: Crawford, F
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Methods Article

Improving image analysis in 2DGE-based redox proteomics by labeling protein carbonyl with fluorescent hydroxylamine

H. Fai Poon¹, Laila Abdullah¹, Jon Reed¹, Sarah M Doore¹, Cyndi Laird¹, Venkat Mathura¹, Michael Mullan¹ and Fiona Crawford¹

¹ Roskamp Institute. Sarasota, FL 34243. USA.

* To whom correspondence should be addressed: H. Fai Poon, Roskamp Institute. 2040 Whitfield Ave., Sarasota, FL 34243. USA. Phone: +941 752 2949. Fax: +941 752 2948. Email: faipoon@rfdn.org

Biol. Proced. Online 2007;9:65-72. doi:10.1251/bpo134
Submitted: August 31, 2007; Accepted: December 09, 2007; Published: December 19, 2007.

Indexing terms: electrophoresis, gel, two-dimensional; oxidation-reduction; protein processing, post-translational.

Abbreviations: FHA, fluorescent hydroxylamine; IEF, isoelectric focusing; MALDI, Matrix-assisted laser desorption/ionization.

Figure 5 Enlarged

Fig. 5:

Representative 2D gel images. (A) Representative 2D gel image of protein carbonyl groups detected by fluorescent hydroxylamine labeling. (B) Representative 2D gel image of protein stained with Coomassie Stain. Numbered spots indicated protein spots used as landmarks for image matching analysis and protein identification by MALDI-MS.

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