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Author: Fernandez, SL
Author: Hurlin, PJ
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Methods Article

Development of human gene reporter cell lines using rAAV mediated homologous recombination

Sandra L. Fernandez1, David W. Russell2 and Peter J. Hurlin3*

1 Shriners Hospitals for Children. Department of Cell Developmental Biology, Oregon Health & Science Portland. OR 97201. USA.
2 Department of Pediatrics, Division of Genetics and Developmental Medicine, University of Washington Seattle. WA 98195-7720. USA.
3 Department of Cell Developmental Biology, Oregon Health & Science Portland. OR 97201. USA. Phone: (503) 221 3438 Fax: (503) 221 3451 Email: pjh@shcc.org.

* To whom correspondence should be addressed: Peter J. Hurlin, Department of Cell Developmental Biology, Oregon Health & Science Portland. OR 97201. USA. Phone: (503) 221 3438. Fax: (503) 221 3451. Email: pjh@shcc.org

Biol. Proced. Online 2007;9:84-90. doi:10.1251/bpo136
Submitted: September 10, 2007; Accepted: November 19, 2007; Published: December 24, 2007.

Indexing terms: Dependovirus; Proto-Oncogene proteins c-myc.

Abbreviations: EGFP, enhanced green fluorescent protein; HFF, human foreskin fibroblast; rAAV, recombinant adeno-associated virus.


Figure 3 Enlarged

Fig. 3:

MR1 and MR2 response to cell cycle entry. MR1 (A) and MR2 (B) clonal populations were subjected to cell cycle entry conditions and then evaluated for EGFP-Luc reporter gene expression. The unstimulated background control populations are shown in the top panels. Serum stimulated populations are shown in the bottom panels where the EGFP-Luciferase expressing cells are located in the P2 gated region.

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