Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

[Abstract] [Full Text] [PDF]

Display

[Abstract]
[Full Text]
PDF

Search in Pubmed

Author: Robenek, H
Author: Severs, NJ
Related works

Methods Article

Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

Horst Robenek¹^* and Nicholas J. Severs²

¹ Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany.
² National Heart and Lung Institute, Imperial College London. U.K..

* To whom correspondence should be addressed: Horst Robenek, Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany. Phone: +49-251-83-56426. Fax: +49-251-83-52998. Email: robenek@uni-muenster.de

Biol. Proced. Online 2008;10:9-19. doi:10.1251/bpo138
Submitted: October 18, 2007; Accepted: December 12, 2007; Published: January 28, 2008.

Indexing terms: Freeze Fracturing; Microscopy, Immunoelectron; Immunogold Techniques.

Figure 3 Enlarged

Fig. 3:

Nomenclature for describing freeze-fracture images of lipid droplets. The diagram shows the phospholipid monolayer surrounding the hydrophobic lipid core. Concavely fractured droplets show an aspect of the monolayer that is equivalent to the P face of a normal membrane. The complementary aspect, revealed in convex fractures, would be termed an E face in a normal lipid bilayer. However, as there is no E half, the "E-face equivalent" seen in convex fractures is best envisaged as the outer-facing aspect of the surface of the lipid core.

Disclaimer/Legal Copyright Privacy Policy