Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

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Author: Robenek, H
Author: Severs, NJ
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Methods Article

Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

Horst Robenek¹^* and Nicholas J. Severs²

¹ Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany.
² National Heart and Lung Institute, Imperial College London. U.K..

* To whom correspondence should be addressed: Horst Robenek, Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany. Phone: +49-251-83-56426. Fax: +49-251-83-52998. Email: robenek@uni-muenster.de

Biol. Proced. Online 2008;10:9-19. doi:10.1251/bpo138
Submitted: October 18, 2007; Accepted: December 12, 2007; Published: January 28, 2008.

Indexing terms: Freeze Fracturing; Microscopy, Immunoelectron; Immunogold Techniques.

Figure 5 Enlarged

Fig. 5:

Similar view to Fig 4. at higher magnification. Gold label for adipophilin is clearly seen on the ER membrane (P Face, PF) immediately adjacent to the lipid droplets (arrows). The lower example arrowed shows a clear demonstration of how the ER membrane curves around the contour of the lipid droplet.

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