Fig. 7:

FRIL images demonstrating that PAT family and other proteins are distributed not only at the lipid droplet surface but also in the cross-fractured lipid droplet core. These examples come from adipocytes and show labeling for perilipin and caveolin-1. (A) Example in which perilipin label is seen predominantly in the outer phospholipid monolayer (P face, PF) of the lipid droplet. (B) Example of a lipid droplet in which caveolin-1 label is seen in the core. (C) Examples in which abundant perilipin label (12nm gold) and simultaneous labeling for caveolin-1 (18 nm gold) and perilipin (12 nm gold) is seen in the core. (D) Example in which abundant caveolin-1 label (18 nm gold) is seen in the core and a mixture of perilipin (12 nm gold) and caveolin-1 (18 nm gold) label is predominantly in the phospholipid monolayer P face (PF).