Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

[Abstract] [Full Text] [PDF]

Display

[Abstract]
[Full Text]
PDF

Search in Pubmed

Author: Robenek, H
Author: Severs, NJ
Related works

Methods Article

Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

Horst Robenek¹^* and Nicholas J. Severs²

¹ Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany.
² National Heart and Lung Institute, Imperial College London. U.K..

* To whom correspondence should be addressed: Horst Robenek, Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany. Phone: +49-251-83-56426. Fax: +49-251-83-52998. Email: robenek@uni-muenster.de

Biol. Proced. Online 2008;10:9-19. doi:10.1251/bpo138
Submitted: October 18, 2007; Accepted: December 12, 2007; Published: January 28, 2008.

Indexing terms: Freeze Fracturing; Microscopy, Immunoelectron; Immunogold Techniques.

Figure 8 Enlarged

Fig. 8:

FRIL images demonstrating that PAT family proteins are present in the plasma membrane, seen in P-face view (PLPF), and undergo profound changes in distribution under conditions of lipid loading of macrophages. (A, B) In the plasma membrane of normal cultured macrophage, adipophilin label is widely distributed throughout the membrane but upon lipid loading, the adipophilin becomes clustered in elevated domains in the plasma membrane. (C, D) Fractures that penetrate beneath the plasma membrane demonstrate that lipid droplets lie beneath the elevated adipophilin-rich plasma membrane domains. Bars: 0.2 µm

Disclaimer/Legal Copyright Privacy Policy