Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

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Author: Robenek, H
Author: Severs, NJ
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Methods Article

Recent advances in freeze-fracture electron microscopy: the replica immunolabeling technique

Horst Robenek¹^* and Nicholas J. Severs²

¹ Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany.
² National Heart and Lung Institute, Imperial College London. U.K..

* To whom correspondence should be addressed: Horst Robenek, Department of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research. University of Münster, Domagkstr. 3D-48149 Münster. Germany. Phone: +49-251-83-56426. Fax: +49-251-83-52998. Email: robenek@uni-muenster.de

Biol. Proced. Online 2008;10:9-19. doi:10.1251/bpo138
Submitted: October 18, 2007; Accepted: December 12, 2007; Published: January 28, 2008.

Indexing terms: Freeze Fracturing; Microscopy, Immunoelectron; Immunogold Techniques.

Figure 9 Enlarged

Fig. 9:

Freeze-fracture view illustrating the structure of a milk fat globule secreted from a human mammary epithelial cell. (A) The globule consists of a lipid droplet core surrounded by a phospholipid monolayer, which in turn is surrounded by a membrane bilayer derived from the plasma membrane which enwraps the droplet during secretion. These different structures are revealed as the fracture plane skips between them (bilayer seen in P face view; phospholipid monolayer in E face view; core seen as lipid is scooped out by cross fracture) (B) FRIL image demonstrating the distribution of adipophilin in the secreted milk fat globule. Abundant label is seen in the bilayer P face (PF). Bars: 0.2 µm

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