Optimized two-dimensional thin layer chromatography to monitor the intracellular concentration of acetyl phosphate and other small phosphorylated molecules

Display

Search in Pubmed

Author: Keating, DH
Author: Wolfe, AJ
Related works

Methods Article

Optimized two-dimensional thin layer chromatography to monitor the intracellular concentration of acetyl phosphate and other small phosphorylated molecules

David H. Keating¹, Ana Shulla¹, Adam H. Klein¹ and Alan J. Wolfe¹^*

¹ Department of Microbiology and Immunology Loyola University Chicago.

* To whom correspondence should be addressed: Alan J. Wolfe, Department of Microbiology and Immunology Loyola University Chicago. 2160 S. First Ave. Bldg. 105 Maywood, IL 60153.. USA. Phone: (708) 216 5814. Fax: (708) 216 9514. Email: awolfe@lumc.edu

Biol. Proced. Online 2008;10:36-46. doi:10.1251/bpo141
Submitted: July 25, 2007; Accepted: December 13, 2007; Published: March 03, 2008.

Indexing terms: Phosphoric Acid Esters; Chromatography, Thin Layer.

Abbreviations: 2D-TLC, two-dimensional thin layer chromatography; acetyl-P, acetyl phosphate; PTA, phosphotransacetylase; PDHC, pyruvate dehydrogenase complex.

Figure 1 Enlarged

Fig. 1:

The PDHC-PTA-ACKA pathway: Glucose and serine (the preferred sugar and amino acid carbon sources of E. coli) are metabolized to pyruvate. The pyruvate is converted to acetyl-CoA through the action of pyruvate dehydrogenase complex (PDHC). The acetyl-CoA is converted to acetyl-P by phosphotransacetylase (PTA) and the acetyl-P is converted to acetate by acetate kinase (ACKA). HS-CoA, coenzyme A; NAD+ and NADH, oxidized and reduced forms of nicotinamide adenine dinucleotide; Pi, inorganic phosphate.

Disclaimer/Legal Copyright Privacy Policy