[Abstract]  [Full Text]  [PDF]

Wim Van Criekinge^1* and Rudi Beyaert²

¹ DEVGEN NV. Technologiepark 9, 9052 Zwijnaarde-Gent. Belgium. Phone: 32-9-264-54-03 Fax: 32-9-264-54-18
² Department of Molecular Biology. Flanders Interuniversity Institute for Biotechnology and University of Ghent, B-9000, Ghent. Belgium.

* To whom correspondence should be addressed: Wim Van Criekinge, DEVGEN NV. Technologiepark 9, 9052 Zwijnaarde-Gent. Belgium. Phone: 32-9-264-54-03. Fax: 32-9-264-54-18. Email: wim.vancriekinge@devgen.com

Biol. Proced. Online 1999;2:1-38. doi:10.1251/bpo16
Submitted: March 22, 1999; Published: October 04, 1999.

Indexing terms: yeasts; hybrid cells.

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination.