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Author: Van Criekinge, W
Author: Beyaert, R
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Review Article

Yeast Two-Hybrid: State of the Art

Wim Van Criekinge1* and Rudi Beyaert2

1 DEVGEN NV. Technologiepark 9, 9052 Zwijnaarde-Gent. Belgium. Phone: 32-9-264-54-03 Fax: 32-9-264-54-18
2 Department of Molecular Biology. Flanders Interuniversity Institute for Biotechnology and University of Ghent, B-9000, Ghent. Belgium.

* To whom correspondence should be addressed: Wim Van Criekinge, DEVGEN NV. Technologiepark 9, 9052 Zwijnaarde-Gent. Belgium. Phone: 32-9-264-54-03. Fax: 32-9-264-54-18. Email: wim.vancriekinge@devgen.com

Biol. Proced. Online 1999;2:1-38. doi:10.1251/bpo16
Submitted: March 22, 1999; Published: October 04, 1999.

Indexing terms: yeasts; hybrid cells.


Figure 1 Enlarged

Fig. 1:

Principle of the Two-hybrid system. (A), (B) Two chimeras, one containing the DNA-binding domain (DB: blue circle) and one that contains an activation domain (AD: half blue circle), are co-transfected into an appropriate host strain. (C) If the fusion partners (yellow and red) interact, the DB and AD are brought into proximity and can activate transcription of reporter genes (here LacZ).

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