Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization

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Author: Abou-Haila, A
Author: Tulsiani, DRP
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Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization

Aida Abou-Haila¹ and Daulat RP Tulsiani²^*

¹ Laboratoire d'Embryologie et Biologie de la Reproduction, UFR Biomédicale. Universite René Descartes, 45 rue des Saints-Pères, 75270 Paris, Cedex 06. France. Email: aida.abou-haila@biomedicale.univ-paris5.fr.
² Departments of Obstetrics & Gynecology and Cell Biology. Vanderbilt University School of Medicine, Room D-3243 MCN, Nashville, TN 37232-2633. USA. Fax: 615/322-4358

* To whom correspondence should be addressed: Daulat Tulsiani, Departments of Obstetrics & Gynecology and Cell Biology. Vanderbilt University School of Medicine, Room D-3243 MCN, Nashville, TN 37232-2633. USA. Fax: 615/322-4358. Email: daulat.tulsiani@mcmail.vanderbilt.edu

Biol. Proced. Online 2001;3:35-42. doi:10.1251/bpo21
Submitted: April 12, 2001; Accepted: November 19, 2001; Published: December 03, 2001.

Indexing terms: acrosome; germ cells; fertilization; glycoside hydrolases.

Abbreviations: MEM, minimum essential medium; BSA, bovine serum albumin; PBS, phosphate buffered saline; SDS, sodium dodecyl sulphate; PAGE, polyacrylamide gel electrophoresis; PNP, p-nitrophenyl; EKRB, enriched Krebs-Ringer bicarbonate.

Figure 3 Enlarged

Fig. 3:

Confocal micrographs showing immunolocalization of β-galactosidase in the forming acrosome during spermiogenesis in the rat. The testicular germ cells were prepared and immunostained using anti-β-galactosidase (monospecific IgG, 5 μg protein/ml) as primary antibody and FITC-labeled anti-rabbit goat IgG as secondary antibody as described in Materials and Methods.

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