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Author: Baliga, NS
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Methods Article

Promoter analysis by saturation mutagenesis

Nitin S Baliga1*

1 Institute for Systems Biology. 4225 Roosevelt way NE, Suite 200, Seattle, WA 98105. USA. Phone: 206-732-1266 Fax: 206-732-1299

* To whom correspondence should be addressed: Nitin Baliga, Institute for Systems Biology. 4225 Roosevelt way NE, Suite 200, Seattle, WA 98105. USA. Phone: 206-732-1266. Fax: 206-732-1299. Email: nbaliga@systemsbiology.org

Biol. Proced. Online 2001;3:64-69. doi:10.1251/bpo24
Submitted: August 30, 2001; Accepted: December 17, 2001; Published: December 22, 2001.

Indexing terms: promoter regions (genetics); mutagenesis; polymerase chain reaction.

Abbreviations: bop , bacterioopsin.


Figure 4 Enlarged

Fig. 4:

Sequence analysis of pNBTATA library plasmids from unscreened (E. coli transformants) vs. screened (Halobacterium SD23 transformants) colonies. A. Tabulation of nucleotide position and identity of the TATA box in seven plasmids prepared from E. coli transformants before the purple/orange screening step. B. Tally of nucleotides (G, A, T, and C) at the seven positions in the TATA box. C. Tally of nucleotides in the TATA box from mutated but active promoters identified through a purple/orange screen.

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