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Author: Baliga, NS
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Methods Article

Promoter analysis by saturation mutagenesis

Nitin S Baliga1*

1 Institute for Systems Biology. 4225 Roosevelt way NE, Suite 200, Seattle, WA 98105. USA. Phone: 206-732-1266 Fax: 206-732-1299

* To whom correspondence should be addressed: Nitin Baliga, Institute for Systems Biology. 4225 Roosevelt way NE, Suite 200, Seattle, WA 98105. USA. Phone: 206-732-1266. Fax: 206-732-1299. Email: nbaliga@systemsbiology.org

Biol. Proced. Online 2001;3:64-69. doi:10.1251/bpo24
Submitted: August 30, 2001; Accepted: December 17, 2001; Published: December 22, 2001.

Indexing terms: promoter regions (genetics); mutagenesis; polymerase chain reaction.

Abbreviations: bop , bacterioopsin.


Figure 6 Enlarged

Fig. 6:

Tabulation of strain designations, promoter sequences, phenotypes, and bacteriorhodopsin content of TATA-box mutants (A) and analysis of the promoter sequences (B). (A) The strain designations are shown in the first column, and sequence in the -31 to -25 nucleotide region (identity to wild type base denoted by a dot), Pum phenotype (-/+/++). (B) The consensus sequence for the TATA-box was determined by counting the appearance of individual nucleotides at each position in Pum+ strains. The consensus sequence is indicated at the bottom with numbers in subscript referring to the percentage of times the most common base(s) were found at that position.

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