High-Yield Method for Isolation and Culture of Endothelial Cells from Rat Coronary Blood Vessels Suitable for Analysis of Intracellular Calcium and Nitric Oxide Biosynthetic Pathways

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Author: Nistri, S
Author: Bani, D
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Methods Article

High-Yield Method for Isolation and Culture of Endothelial Cells from Rat Coronary Blood Vessels Suitable for Analysis of Intracellular Calcium and Nitric Oxide Biosynthetic Pathways

Silvia Nistri¹, Luca Mazzetti², Paola Failli² and Daniele Bani¹^*

¹ Department of Anatomy, Histology and Forensic Medicine, Section of Histology, University of Florence, Italy. V. le G. Pieraccini, 6, I-50139 Florence. Italy. Fax: (+39)055 4271385
² Department of Preclinical and Clinical Pharmacology, University of Florence, Italy. V. le G. Pieraccini, 6, I-50139 Florence. Italy.

* To whom correspondence should be addressed: Daniele Bani, Department of Anatomy, Histology and Forensic Medicine, Section of Histology, University of Florence, Italy. V. le G. Pieraccini, 6, I-50139 Florence. Italy. Fax: (+39)055 4271385. Email: daniele.bani@unifi.it

Biol. Proced. Online 2002;4:32-37. doi:10.1251/bpo31
Submitted: May 30, 2002; Accepted: August 15, 2002; Published: October 28, 2002.

Indexing terms: nitric oxide; Fura-2.

Figure 2 Enlarged

Fig. 2:

Phase-contrast microscopy of putative RCE cells adherent to the floor of a 25 cm2 flask 4 h after seeding. Most of them appear as small-sized, round-shaped bodies. A few cells already show a more flattened shape (arrow). Bar = 100 μm

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