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Author: Nistri, S
Author: Bani, D
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Methods Article

High-Yield Method for Isolation and Culture of Endothelial Cells from Rat Coronary Blood Vessels Suitable for Analysis of Intracellular Calcium and Nitric Oxide Biosynthetic Pathways

Silvia Nistri1, Luca Mazzetti2, Paola Failli2 and Daniele Bani1*

1 Department of Anatomy, Histology and Forensic Medicine, Section of Histology, University of Florence, Italy. V. le G. Pieraccini, 6, I-50139 Florence. Italy. Fax: (+39)055 4271385
2 Department of Preclinical and Clinical Pharmacology, University of Florence, Italy. V. le G. Pieraccini, 6, I-50139 Florence. Italy.

* To whom correspondence should be addressed: Daniele Bani, Department of Anatomy, Histology and Forensic Medicine, Section of Histology, University of Florence, Italy. V. le G. Pieraccini, 6, I-50139 Florence. Italy. Fax: (+39)055 4271385. Email: daniele.bani@unifi.it

Biol. Proced. Online 2002;4:32-37. doi:10.1251/bpo31
Submitted: May 30, 2002; Accepted: August 15, 2002; Published: October 28, 2002.

Indexing terms: nitric oxide; Fura-2.


Figure 6 Enlarged

Fig. 6:

Representative experiment with RCE cells loaded simultaneously with DAF-2/DA and fura-2AM showing the time-course of intracellular NO (left value axis & red curve) and Ca2+ (right value axis & black curve).

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