Use of an Immobilized Monoclonal Antibody to Examine Integrin α5β1 Signaling Independent of Cell Spreading

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Author: Bao, W
Author: Strömblad, S
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Use of an Immobilized Monoclonal Antibody to Examine Integrin α5β1 Signaling Independent of Cell Spreading

Wenjie Bao¹^* and Staffan Strömblad¹

¹ Karolinska Institutet, Department of Microbiology, Pathology and Immunology. Huddinge University Hospital F46, SE-141 86 Huddinge. Sweden. Phone: 46-8-58581036 Fax: 46-8-58581020

* To whom correspondence should be addressed: Wenjie Bao, Karolinska Institutet, Department of Microbiology, Pathology and Immunology. Huddinge University Hospital F46, SE-141 86 Huddinge. Sweden. Phone: 46-8-58581036. Fax: 46-8-58581020. Email: wenjie.bao@impi.ki.se

Biol. Proced. Online 2002;4:81-87. doi:10.1251/bpo37
Submitted: September 12, 2002; Accepted: October 15, 2002; Published: November 11, 2002.

Indexing terms: cell adhesion; integrins; antibodies, monoclonal; fibronectins; protein kinases.

Figure 1 Enlarged

Fig. 1:

Cell morphology was observed upon attachment onto immobilized anti-integrin mabs. Photographs (20´ objective) show representative morphology of ECV 304 cells after attachment onto dishes pre-coated with fibronectin (FN), anti-integrin mabs JBS5 (anti-α5β1), P4C10 (anti-β1) or LM609 (anti-αvβ3) and poly-L-lysin (p-L-L) for 30 min.

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