A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

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Author: Nunemaker, CS
Author: Moenter, SM
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A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

Craig S. Nunemaker¹, R. Anthony DeFazio¹ and Suzanne M. Moenter¹^*

¹ Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA.

* To whom correspondence should be addressed: Suzanne M. Moenter, Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA. Phone: (434) 982-0076. Fax: (434) 982-0088. Email: smm4n@virginia.edu

Biol. Proced. Online 2003;5:53-62. doi:10.1251/bpo46
Submitted: December 17, 2002; Accepted: February 05, 2003; Published: February 17, 2003.

Indexing terms: gonadorelin; periodicity; neurons.

Figure 1 Enlarged

Fig. 1:

Recording pipette approaching a cell for targeted extracellular recording. Sketch showing the relationship between a brain slice containing the target cell and the recording pipette. The arrows illustrate the path the pipette should move to enter the slice and approach the cell. The sawing motion is critical for long-term stability as this avoids compression of tissue under the electrode.

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