A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

Display

Search in Pubmed

Author: Nunemaker, CS
Author: Moenter, SM
Related works

Methods Article

A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

Craig S. Nunemaker¹, R. Anthony DeFazio¹ and Suzanne M. Moenter¹^*

¹ Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA.

* To whom correspondence should be addressed: Suzanne M. Moenter, Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA. Phone: (434) 982-0076. Fax: (434) 982-0088. Email: smm4n@virginia.edu

Biol. Proced. Online 2003;5:53-62. doi:10.1251/bpo46
Submitted: December 17, 2002; Accepted: February 05, 2003; Published: February 17, 2003.

Indexing terms: gonadorelin; periodicity; neurons.

Figure 2 Enlarged

Fig. 2:

Differences between low-resistance (loose) and tight seal recordings. (A-B) a 10-sec digitized recording segment displaying both the baseline trace and downward vertical deflections indicating action currents (events). (A) With the loose seal (16 MΩ), event amplitude is ~200 pA and peak-to-peak noise ~25 pA, resulting is a signal-to-noise ratio of 8:1. In some low resistance seals, this ratio can exceed 50:1. (B) With the tight seal (GΩ, traditional cell-attached configuration), event amplitude is ~40 pA and peak-to-peak noise ~20 pA, resulting in a lower signal-to-noise ratio of 2:1. Note that A and B are recordings from two different cells, representative of the general finding using each technique.

Disclaimer/Legal Copyright Privacy Policy

ISSN: 1480-9222
Article © by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2007 Biological Procedures Online.