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Author: Nunemaker, CS
Author: Moenter, SM
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Methods Article

A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

Craig S. Nunemaker1, R. Anthony DeFazio1 and Suzanne M. Moenter1*

1 Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA.

* To whom correspondence should be addressed: Suzanne M. Moenter, Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA. Phone: (434) 982-0076. Fax: (434) 982-0088. Email: smm4n@virginia.edu

Biol. Proced. Online 2003;5:53-62. doi:10.1251/bpo46
Submitted: December 17, 2002; Accepted: February 05, 2003; Published: February 17, 2003.

Indexing terms: gonadorelin; periodicity; neurons.


Figure 3 Enlarged

Fig. 3:

A long-term recording using the targeted extracellular technique. Firing rate of a GnRH neuron recorded in a coronal brain slice is displayed at 1-min intervals. Vertical bars above this graph indicate the time of occurrence for each action current composing the firing rate plot. * indicate episodes of significant changes in firing rate detected by CLUSTER7 pulse detection algorithm. We believe the changes in action current amplitude are due to shifts in the cell location with respect to the pipette tip and to changes in seal resistance. Data adapted from (7) with permission.

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