A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

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Author: Nunemaker, CS
Author: Moenter, SM
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A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

Craig S. Nunemaker¹, R. Anthony DeFazio¹ and Suzanne M. Moenter¹^*

¹ Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA.

* To whom correspondence should be addressed: Suzanne M. Moenter, Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing, University of Virginia. P.O. Box 800578, Jefferson Park Avenue, University of Virginia, Charlottesville, VA 22908. USA. Phone: (434) 982-0076. Fax: (434) 982-0088. Email: smm4n@virginia.edu

Biol. Proced. Online 2003;5:53-62. doi:10.1251/bpo46
Submitted: December 17, 2002; Accepted: February 05, 2003; Published: February 17, 2003.

Indexing terms: gonadorelin; periodicity; neurons.

Figure 4 Enlarged

Fig. 4:

Examples of the termination of a recording. Recordings were made of a GFP-expressing GnRH neuron in a coronal brain slice. (A) An example of the decrease in amplitude that can occur when a low resistance seal deteriorates. (B) An example of a quiescent cell, in which no events were detected until stimulated after by bath application of 10-15 mM KCl. (C) An example of failed seal, in which bath application of 10-15 mM KCl did not evoke detectable events.

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