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Author: Malloff, C
Author: Lam, W
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Methods Article

Two-dimensional DNA displays for comparisons of bacterial genomes

Chad Malloff1, Edie Dullaghan2, Alice Li3, Richard Stokes4, Rachel Fernandez5* and Wan Lam1

1 Departments of Pathology and Laboratory Medicine. British Columbia Cancer Research Center. Vancouver, BC. Canada.
2 Department of Pediatrics and the Division of Infectious and Immunological Diseases. British Columbia Children's Hospital. Vancouver, BC. Canada.
3 Departments of Pathology and Laboratory Medicine. The Division of Infectious and Immunological Diseases. British Columbia Children's Hospital. Vancouver, BC. Canada.
4 Departments of Pathology and Laboratory Medicine, Pediatrics, Microbiology and Immunology. University of British Columbia. The Division of Infectious and Immunological Diseases. British Columbia Children's Hospital. Vancouver, BC. Canada.
5 Microbiology and Immunology. University of British Columbia. Vancouver, BC. Canada.

* To whom correspondence should be addressed: Rachel Fernandez, Department of Microbiology & Immunology University of British Columbia. Vancouver, BC. Canada. Phone: (604) 822-6824. Fax: (604) 822-6041. Email: rachelf@interchange.ubc.ca

Biol. Proced. Online 2003;5:143-152. doi:10.1251/bpo56
Submitted: March 16, 2003; Accepted: April 01, 2003; Published: June 15, 2003.

Indexing terms: Genomics; DNA fingerprinting.


Figure 1 Enlarged

Fig. 1:

Schematic outlining Two-dimensional Bacterial Genomic Display. (a) Samples to be compared are digested with the same frequent cutting enzyme(s) and radiolabelled (asterisk). (b) The fragments are then separated by size on the same first dimension polyacrylamide gel. (c) First dimension gel lanes are cut from the gel and transferred to the top of identical denaturing gradient polyacrylamide gels. (d) Sample fragments are then resolved in the second dimension based on melting characteristics. (e) The gel is then washed and dried before exposure to autoradiography film to generate an image for comparison (f).

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