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Author: Malloff, C
Author: Lam, W
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Methods Article

Two-dimensional DNA displays for comparisons of bacterial genomes

Chad Malloff1, Edie Dullaghan2, Alice Li3, Richard Stokes4, Rachel Fernandez5* and Wan Lam1

1 Departments of Pathology and Laboratory Medicine. British Columbia Cancer Research Center. Vancouver, BC. Canada.
2 Department of Pediatrics and the Division of Infectious and Immunological Diseases. British Columbia Children's Hospital. Vancouver, BC. Canada.
3 Departments of Pathology and Laboratory Medicine. The Division of Infectious and Immunological Diseases. British Columbia Children's Hospital. Vancouver, BC. Canada.
4 Departments of Pathology and Laboratory Medicine, Pediatrics, Microbiology and Immunology. University of British Columbia. The Division of Infectious and Immunological Diseases. British Columbia Children's Hospital. Vancouver, BC. Canada.
5 Microbiology and Immunology. University of British Columbia. Vancouver, BC. Canada.

* To whom correspondence should be addressed: Rachel Fernandez, Department of Microbiology & Immunology University of British Columbia. Vancouver, BC. Canada. Phone: (604) 822-6824. Fax: (604) 822-6041. Email: rachelf@interchange.ubc.ca

Biol. Proced. Online 2003;5:143-152. doi:10.1251/bpo56
Submitted: March 16, 2003; Accepted: April 01, 2003; Published: June 15, 2003.

Indexing terms: Genomics; DNA fingerprinting.


Figure 5 Enlarged

Fig. 5:

Practical aspects of 2DDE. (a) Preparation of glass plates. (b) Apparatus used to simultaneously pour 24 denaturing gradient gels. (c) Preparing 1st dimension for transfer. (d) Transfer of 1D strip to 2D gel. (e) Simultaneous running of multiple gels.

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