Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects

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Author: Mahajan, SD
Author: Nair, MP
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Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects

Supriya D. Mahajan¹^*, Ravikumar Aalinkeel¹, Stanley A. Schwartz¹, Ram P. Chawda¹ and Madhavan P.N. Nair¹

¹ Department of Medicine, Div of Allergy, Immunology, and Rheumatology. 310 Multi Research Bldg., Buffalo General Hospital, 100 High Street, Buffalo, NY 14203. USA.

* To whom correspondence should be addressed: Supriya D. Mahajan, Dr. Supriya Mahajan Ph.D., Research Assistant Professor, Department of Medicine, Div of Allergy, Immunology, and Rheumatology. 310 Multi Research Bldg., Buffalo General Hospital, 100 High Street, Buffalo, NY 14203. USA. Phone: 716-859-2985. Fax: 716-859-2999. Email: smahajan@buffalo.edu

Biol. Proced. Online 2003;5:182-188. doi:10.1251/bpo60
Submitted: June 05, 2003; Accepted: August 26, 2003; Published: September 05, 2003.

Indexing terms: CD8-Positive T-Lymphocytes; HIV; Killer Cells, Natural.

Abbreviations: CTL, Cytotoxic T lymphocyte; HIV, Human immunodeficiency virus; NK, natural killer; PI, propidium iodide; 7AAD, 7-aminoactinomycin D; PBMCs, peripheral blood mononuclear cells; E:T, Effector: Target; R1/R2, Region 1/Region 2; FL-1, fluorescence axis 1; FL-2, fluorescence axis 2; SSC, side scatter; PE, phycoerythrin.

Figure 1 Enlarged

Fig. 1:

Shows R1 gate around the Target cells, and R2 gate around the Effector cells. The Target cells are labeled with PKH 67 and represented on the Y axis (FL-1), X-axis represents side scatter.

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