[Abstract]  [Full Text]  [PDF]

Display
[Abstract]
[Full Text]
PDF
Search in Pubmed
Author: Mahajan, SD
Author: Nair, MP
Related works

Methods Article

Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects

Supriya D. Mahajan1*, Ravikumar Aalinkeel1, Stanley A. Schwartz1, Ram P. Chawda1 and Madhavan P.N. Nair1

1 Department of Medicine, Div of Allergy, Immunology, and Rheumatology. 310 Multi Research Bldg., Buffalo General Hospital, 100 High Street, Buffalo, NY 14203. USA.

* To whom correspondence should be addressed: Supriya D. Mahajan, Dr. Supriya Mahajan Ph.D., Research Assistant Professor, Department of Medicine, Div of Allergy, Immunology, and Rheumatology. 310 Multi Research Bldg., Buffalo General Hospital, 100 High Street, Buffalo, NY 14203. USA. Phone: 716-859-2985. Fax: 716-859-2999. Email: smahajan@buffalo.edu

Biol. Proced. Online 2003;5:182-188. doi:10.1251/bpo60
Submitted: June 05, 2003; Accepted: August 26, 2003; Published: September 05, 2003.

Indexing terms: CD8-Positive T-Lymphocytes; HIV; Killer Cells, Natural.

Abbreviations: CTL, Cytotoxic T lymphocyte; HIV, Human immunodeficiency virus; NK, natural killer; PI, propidium iodide; 7AAD, 7-aminoactinomycin D; PBMCs, peripheral blood mononuclear cells; E:T, Effector: Target; R1/R2, Region 1/Region 2; FL-1, fluorescence axis 1; FL-2, fluorescence axis 2; SSC, side scatter; PE, phycoerythrin.


Figure 3 Enlarged

Fig. 3:

Representative FACS Analysis data from normal and HIV patient showing granzyme B expression. A: Shows Isotype control (IgGk1) used for setting the regions. Figure B a, b, c, d and Figure C a, b, c, d shows the percentage of Granzyme B release from the effector cells of normal controls and the HIV patients using an R2 gate after the 4 hr cytotoxicity assay with E: T ratios of 1:1, 10:1, 25:1 and 50:1 respectively. Percent Granzyme B expression is represented on the FL-2 axis (PE).

Disclaimer/Legal Copyright Privacy Policy

ISSN: 1480-9222
Article © by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2007 Biological Procedures Online.