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Author: Mahajan, SD
Author: Nair, MP
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Methods Article

Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects

Supriya D. Mahajan1*, Ravikumar Aalinkeel1, Stanley A. Schwartz1, Ram P. Chawda1 and Madhavan P.N. Nair1

1 Department of Medicine, Div of Allergy, Immunology, and Rheumatology. 310 Multi Research Bldg., Buffalo General Hospital, 100 High Street, Buffalo, NY 14203. USA.

* To whom correspondence should be addressed: Supriya D. Mahajan, Dr. Supriya Mahajan Ph.D., Research Assistant Professor, Department of Medicine, Div of Allergy, Immunology, and Rheumatology. 310 Multi Research Bldg., Buffalo General Hospital, 100 High Street, Buffalo, NY 14203. USA. Phone: 716-859-2985. Fax: 716-859-2999. Email: smahajan@buffalo.edu

Biol. Proced. Online 2003;5:182-188. doi:10.1251/bpo60
Submitted: June 05, 2003; Accepted: August 26, 2003; Published: September 05, 2003.

Indexing terms: CD8-Positive T-Lymphocytes; HIV; Killer Cells, Natural.

Abbreviations: CTL, Cytotoxic T lymphocyte; HIV, Human immunodeficiency virus; NK, natural killer; PI, propidium iodide; 7AAD, 7-aminoactinomycin D; PBMCs, peripheral blood mononuclear cells; E:T, Effector: Target; R1/R2, Region 1/Region 2; FL-1, fluorescence axis 1; FL-2, fluorescence axis 2; SSC, side scatter; PE, phycoerythrin.


Figure 5 Enlarged

Fig. 5:

Representative histogram of the percent CD8 and CD69 (NK cells) expression in the effector cells in HIV subjects and normal controls as measured by flow cytometry. Data presented is the mean + SD of 3 experiments where the total of 10 subjects in each group.

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