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Author: Li, J
Author: McManus, DP
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Methods Article

Recombinant antigens for immunodiagnosis of cystic echinococcosis

Jun Li1, Wen-Bao Zhang1 and Donald P. McManus1*

1 Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia.

* To whom correspondence should be addressed: Donald P. McManus, Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia. Phone: 61-7-3362 0401. Fax: 61-7-3362 0104. Email: donM@qimr.edu.au

Biol. Proced. Online 2004;6:67-77. doi:10.1251/bpo74
Submitted: April 12, 2004; Accepted: April 27, 2004; Published: May 10, 2004.

Indexing terms: Echinococcus; Recombinant proteins; Blotting, Western.

Abbreviations: CE, cystic echinococcosis; AE, alveolar echinococcosis; NCC, neurocysticercosis; Schist, schistosomiasis; Others, other infections/clinical presentations; Healthy, uninfected controls.


Figure 1 Enlarged

Fig. 1:

Western blot analysis showing the recognition of hydatid cyst fluid (HCF) antigens of Echinococcus granulosus by different sources of sera. Lane M, protein marker; Lane 1, pooled sera from normal mice; Lane 2, pooled sera from mice experimentally infected with oncospheres of E. granulosus; Lane 3, pooled sera from mice infected with Plasmodium yoelii; Lane 4, pooled sera from mice infected with Schistosoma japonicum; Lane 5, pooled normal human sera; Lane 6, pooled sera from humans with proven cystic hydatid disease infection; Lane 7, pooled sera from humans with proven alveolar echinococcosis infection; Lane 8, pooled sera from humans with proven neurocysticercosis infection; Lane 9, hyperimmune rabbit serum prepared against hydatid cyst fluid antigens. HCF antigen B (AgB) is arrowed. Protein standard markers are shown on the left hand of the figure.

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