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Author: Li, J
Author: McManus, DP
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Methods Article

Recombinant antigens for immunodiagnosis of cystic echinococcosis

Jun Li1, Wen-Bao Zhang1 and Donald P. McManus1*

1 Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia.

* To whom correspondence should be addressed: Donald P. McManus, Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia. Phone: 61-7-3362 0401. Fax: 61-7-3362 0104. Email: donM@qimr.edu.au

Biol. Proced. Online 2004;6:67-77. doi:10.1251/bpo74
Submitted: April 12, 2004; Accepted: April 27, 2004; Published: May 10, 2004.

Indexing terms: Echinococcus; Recombinant proteins; Blotting, Western.

Abbreviations: CE, cystic echinococcosis; AE, alveolar echinococcosis; NCC, neurocysticercosis; Schist, schistosomiasis; Others, other infections/clinical presentations; Healthy, uninfected controls.


Figure 2 Enlarged

Fig. 2:

Size of cDNA synthesised from mRNA isolated from protoscoleces of E. granulosus. After the second-strand cDNA was synthesised by adding 2μl of [α-32P] dATP to the reaction, the cDNA was fractioned through a CL-2B column after being linked with EcoR I adapters and digested with Xho I to create an Xho I cut end. Fractions 5 to 11 (1μl of elution) were run on an agarose gel and exposed for 4 h to an X-ray film before being developed. Standard RNA markers, in nucleotides are shown on the left hand of the figure.

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