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Author: Li, J
Author: McManus, DP
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Methods Article

Recombinant antigens for immunodiagnosis of cystic echinococcosis

Jun Li1, Wen-Bao Zhang1 and Donald P. McManus1*

1 Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia.

* To whom correspondence should be addressed: Donald P. McManus, Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia. Phone: 61-7-3362 0401. Fax: 61-7-3362 0104. Email: donM@qimr.edu.au

Biol. Proced. Online 2004;6:67-77. doi:10.1251/bpo74
Submitted: April 12, 2004; Accepted: April 27, 2004; Published: May 10, 2004.

Indexing terms: Echinococcus; Recombinant proteins; Blotting, Western.

Abbreviations: CE, cystic echinococcosis; AE, alveolar echinococcosis; NCC, neurocysticercosis; Schist, schistosomiasis; Others, other infections/clinical presentations; Healthy, uninfected controls.


Figure 3 Enlarged

Fig. 3:

The cDNA insert sizes of randomly selected clones from the protoscolex cDNA library. The cDNA insert sizes were determined by PCR using T3 and T7 primers with the PCR products being electrophorezed on a 1% (w/v) agarose gel. The size of the DNA fragments (Lanes 1-21) were compared with standard DNA ladder (Gibco BRL) (Lane M).

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