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Author: Li, J
Author: McManus, DP
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Methods Article

Recombinant antigens for immunodiagnosis of cystic echinococcosis

Jun Li1, Wen-Bao Zhang1 and Donald P. McManus1*

1 Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia.

* To whom correspondence should be addressed: Donald P. McManus, Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research. 300 Herston Road, Herston, Brisbane, Queensland 4029. Australia. Phone: 61-7-3362 0401. Fax: 61-7-3362 0104. Email: donM@qimr.edu.au

Biol. Proced. Online 2004;6:67-77. doi:10.1251/bpo74
Submitted: April 12, 2004; Accepted: April 27, 2004; Published: May 10, 2004.

Indexing terms: Echinococcus; Recombinant proteins; Blotting, Western.

Abbreviations: CE, cystic echinococcosis; AE, alveolar echinococcosis; NCC, neurocysticercosis; Schist, schistosomiasis; Others, other infections/clinical presentations; Healthy, uninfected controls.


Figure 5 Enlarged

Fig. 5:

Expression, purification (Panel A, SDS-PAGE stained by Coomassie blue) and Western blotting of recombinant proteins for diagnosis of cystic echinococcosis (CE) with a pool of sera from patients confirmed with CE (Panel B), and a pool of sera from mice experimentally infected with oncospheres of E. granulosus (Panel C). Lane El, bacterial lysate containing EpC1-GST; Lane Ep, affinity purified EpC1-GST; Lane Tl, bacterial lysate containing EgTPx-GST; Lane Tp, affinity purified EgTPx-GST; Lane Gl, bacterial lysate containing EgG5-GST; Lane Gp, affinity purified EgG5-GST. Protein standard markers (in kDa) are shown on the left side of the panels.

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