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Author: Pick, N
Author: Av-Gay, Y
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Methods Article

Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry

Neora Pick1, Scott Cameron1, Dorit Arad1 and Yossef Av-Gay1*

1 University of British Columbia, Department of Medicine, Division of Infectious Diseases. Vancouver, BC, V5Z 3J5. Canada.

* To whom correspondence should be addressed: Yossef Av-Gay, University of British Columbia, Department of Medicine, Division of Infectious Diseases. 2733 Heather Street, D450 HP East, Vancouver, BC, V5Z 3J5. Canada. Phone: 604-875-4588. Email: yossi@interchange.ubc.ca

Biol. Proced. Online 2004;6:220-225. doi:10.1251/bpo92
Submitted: February 25, 2004; Accepted: September 19, 2004; Published: October 01, 2004.

Indexing terms: Flow cytometry; Propidium; Lethal Dose 50.

Abbreviations: FACS, Fluorescence Activated Cell Sorting; PI, propidium iodide; LD50, lethal dose in which 50% of the cells die.


Figure 1 Enlarged

Fig. 1:

Effect of Compound treatment on THP-1 cell membrane permeability as measured by PI exclusion staining. Linear forward scatter vs Log side scatter dot plots (A) histograms of PI stain intensity detected on the FL3 channel (B) of THP-1 cells treated for 24 hours with a lead compound being tested for drug toxicity. (I) Healthy THP-1 cells in the log phase of growth, treated with drug carrier (4% methanol in culture medium), and stained with PI as outlined. 10.9% are dead as determined by the PI gate. This correlates well with the indicated dead cell gate based on changes in forward and side scatter. (II) THP-1 cells treated with 50 μg/ml of the test compound, 23.7% are PI positive. (III) THP-1 cells treated with 100 μg/ml of the test compound, 47.6% are PI positive. (IV) THP-1 cells treated with 200 μg/ml of the test compound, 95.3% are PI positive = dead cells.

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