NEW PUBLISHER We are pleased to announce that Springer Science and Business Media will now publish Biological Procedures Online. Springer brings to our journal a larger, stronger infrastructure, a greater number of skilled scientists and a higher visibility than ever before. With these changes our impact factor of 1.179 can only go in one direction: that's even higher.

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BPO is an open access-based journal of research methods in the medical and biological sciences. Papers feature step-by-step protocols to help you work quickly and correctly.

Indexed by: Biological Abstracts, Biotechnology Citation Index, Biosis Previews, Chemical Abstracts, EMBASE, ISI Web of Science, PubMed Central, Science Citation Index Expanded, and the Zoological Record. Archived by PubMed Central and the National Library of Canada.

Impact Factor = 1.179: This is the Impact Factor for Biological Procedures Online as listed in ISI's 2007 Journal Citation Reports. A list of citations can be accessed through Google Scholar.

A method to generate human mesenchymal stem cell-derived meurons which express and are excited by multiple neurotransmitters (November 27/08)

A protocol to generate heterogenous populations of neurotransmitter-producing neurons from human mesencymal stem cells.

A rapid microwell fluorescence immunoassay for cellular protein detection (September 22/08)

A simple fluorescence immunoassay for the detection of proteins directly on cells cultured in 96-well plates. It is useful for detecting poorly expressed proteins and for drug development.

Tracking and ablating subpopulations of epiblast cells in the chick embryo (September 1/08)

A protocol to examine chick embryo cells during development to label them for cell tracking purposes.

Characterizing gene family evolution (June 20/08)

The traditional use of concepts such as monophyly and the dichotomy between homoplasty and homology as applied to gene families are critiqued. The utility of layers of homology and the meaning of homology at the character state level in the context of sequence evolution is questioned.

Cell culture-based analysis of postsynaptic membrane assembly in muscle cells (May 23/08)

A method for studying postsynaptic membrane assembly using replated cultures of aneural differentiated skeletal muscle cells. This method is validated for biochemical and microscopy studies.

A method for purification, identification and validation of DNMT1 mRNA binding proteins (Mar 28/08)

Different approaches are discussed to isolate and identify a RNA binding protein and how to validate its role. The protein is DNA methyltransferase 1, an enzyme responsible for the maintenance of DNA methylation patterns during cell division.

Optimized two-dimensional thin layer chromatography to monitor the intracellular concentration of acetyl phosphate and other small phosphorylated molecules (Mar 3/08)

A protocol that results in greatly improved reproducibility and permits precise measurements of the concentrations of intracellular acetyl-P and other small phosphorylated molecules.

Identification of a Calcitriol-Regulated Sp-1 Site in the Promoter of Human CD14 using a Combined Western Blotting Electrophoresis Mobility Shift Assay (WEMSA) (Feb 17/08)

A technique that does not require oligos that can be broadly applied to aid in the identification of transcription factors involved in regulating gene expression.

Quantitative Evaluation of Signaling Events in Drosophila S2 Cells (Feb 4/08)

A simple method for the quantification of c-Jun N-terminal kinase activation by Western blot analysis or in tissue culture plates. The In-Cell method described here is easily extended to quantification of unrelated signaling events.

Recent Advances in Freeze-Fracture Electron Microscopy: The Replica Immunolabeling Technique (Jan 28/08)

Freeze-fracture replica immunogold labeling permits the location of target molecules within the cell membrane to be determined. Applications of lipid droplet biogenisis and function are discussed.

In vivo and in vitro techniques for comparative study of antiviral T-cell responses in the amphibian Xenopus (Jan 17/08)

BrdU incorporation and CFSE dilution assays are reviewed in detail to quantitate proliferative responses of splenocytes to viral infection in Xenopus.

Development of human gene reporter cell lines using rAAV mediated homologous recombination (Dec 24/07)

A novel method for generating human cell lines that serve as reporters of transcriptional activity using recombinant adeno-associated virus to mediate the insertion of exogenous DNA sequences into specific genomic loci through homologous recombination.

Micro-scale flow cytometry-based and biochemical analysis of lipid signaling in primary B cell subpopulations (Dec 24/07)

Mini-scale protocols to assess lipid signalling with as few as 0.5x106 purified early (T1) and late (T2) transitional B cells.

Route to obesity passes through tongue

Secret to workplace happiness? Remember what you love about the job, study urges

Using water to understand human society, from the industrial revolution to global trade

Scientists at CSHL uncover new RNA processing mechanism and a new class of small RNAs

'Junk' DNA proves functional

New regulatory mechanism discovered for cell identify and behavior in forming organs

Nitric oxide can alter brain function

Scientists Present 'Moving' Theory Behind Bacterial Decision-Making

Superglue from the sea

Updated: January 24/09

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